Always DIY; never Detransition

Okay, so everything is pretty fucked: access is blocked, prescriptions are hard to get, insurance is scarce, and generics are sold out, so things get expensive. Yes yes, quite fucked all the way around. So, let’s skip a few steps, and just make it and give it away for free, shall we?

Let’s rock.

In the event that you aren’t looking to make them from scratch, but rather you got one of our vials [or would like to] we shall address the basic questions everyone asks when coming across our stuff: What is it? How do I use it? How do I know it’s safe?

We’ll address these questions and more, just read on. Instructions for making it yourself are a bit more involved, and will be forthcoming.

What is it?
This is Testosterone enanthate (A.K.A. TEn), it was chosen for a handful of reasons. Many people use propionate or cypionate, but enanthate is used outside the US for HRT quite commonly. The vial contains roughly 10 months worth of HRT depending on how you dose. It can be injected either intramuscularly or subcutaneously. Details on that in the next section.

The specific formula of this injectable was selected for easy distribution and long shelf life. If you have other means to access HRT medication, we urge using those first, and giving ours to those who are blocked from access. 

Let’s make the application of testosterone as safe and convenient as possible for anyone who wants it.


This particular formulation is best administered using intramuscular injection into the thigh once a week. There is plenty of information on how to self-administer IM medication elsewhere on the internet. The following highlights info that is specific to 200mg/ml Testosterone Enanthate.

There are a few things which are important to keep in mind. First is that since it’s almost a year’s worth, you’re going to have to be extremely vigilant about keeping your vial uncontaminated. Being careful about this is just a matter of paying attention, and making it a ritual of sorts, so that you do it the same every time. It should go without saying, but don’t dose if you’re wasted or exhausted; just wait until tomorrow.* Designate a place where you’ll do it, and make a surface which is easy to clean. Either you can go nuts, and use a sterile drape, or you can just use something non-porous like aluminum foil [shiny side up] or plastic wrap which you can wipe down with alcohol, or chlorhexidine.

Check the vial for any contamination. Small air bubbles are normal, any solids or strings floating in solution are signs for contamination. Do not use a contaminated vial. Wipe down the top of the vial using an alcohol or chlorhexidine pad thoroughly and carefully before every use. Again: given that these vials are designed to be used many times, they require more care to avoid contamination. Using a 23G or 25G needle attached to a 1ml syringe, withdraw 0.25ml of solution.

Remove the needle used for withdrawing and swap it for a 25G or 27G, 1″ or 1-1/2″ needle. If you don’t have easy access to needles, you can use the same needle for both withdrawing & injecting, it will just be less comfortable. Never reuse or share needles.

Since the concentration of testosterone is fairly high, it is important to have good control over the small amount of liquid you are injecting. Be aware of dead volume in the tip of the syringe and the needle itself. Holding the syringe needle-up, push in the plunger until you see a drop of liquid forming at the tip of the injection needle. Whatever volume is left in the syringe after priming is the amount of liquid that will end up in your body. You will see that you actually need to withdraw 0.3 – 0.35ml of liquid in order to deliver 0.25ml of Testosterone. Administer the injection into your muscle tissue at least 1 inch (5cm) deep.

After use, we recommend putting the vial cap back on to protect the diaphragm from dust between injections. Store the vial in a dark place at room temperature. If you think your room might get above 25°C (77°F) during summer, store the vial in a fridge and remove it 2-3h before use (you can also just do this anyway). If precipitate crystals fall out of solution from the cold, warm the vial in your hands until they dissolve; this will not affect the efficacy of the solution.


These were manufactured in a cleanroom microfactory. That’s why they look like they came from a factory: they did. Particulate count in the air is continuously monitored, the active pharmaceutical ingredient of every batch is tested for purity and composition, and the oils, preservatives, and substrates are all US pharmacopia grade ingredients. The vials and caps are sterilized in an autoclave, and are filled under a laminar flow hood fed from a HEPA filter. Details of sterile compounding technique will be covered in the entry on how to set up production yourself.

Please don’t hoard these: despite the fact that they will keep for the full year that it will take to use them up, they won’t keep for three years, so if you take a bunch in hopes of doomsday prepping, or having a decade’s worth, it’s not going to work. Just share them, and we can work on getting you more next year, okay?

IM vs subQ: Testosterone enanthate can be administered both intramuscularly and subcutaneously. While many people prefer “subQ” injections, we recommend starting with IM injection. The 200 mg/ml Testosterone Enanthate contains a high concentration of benzyl alcohol as a preservative (2%). This makes distribution easier and allows for a longer period of safe use after the vial is opened. Unfortunately, benzyl alcohol is known to sometimes cause discomfort at the injection site. However, most of these effects only occur in the skin layer and do not cause issues if the injection is applied into muscle tissue, hence our recommendation for IM. This is different for everyone, so we encourage you to try both, and determine which injection works better for you, since both provide the same absorption rate into the bloodstream.

Testosterone Enanthate: When testosterone dissolved in oil is injected into tissue, it forms a “depo”, and testosterone is slowly absorbed into the bloodstream. Depending on what specific oil and testosterone ester is used, the rate of absorption can vary widely. The most common testosterone ester used for injections in the United States is Testosterone Cypionate (Depo-Testosterone). While cypionate has an acceptable half-life for weekly injections, propionate is known to cause rapid fluctuation of circulating testosterone levels and is typically injected twice a week. A longer half-life contributes to more stable levels and reduces the occurrence of mood swings.

This injection uses Testosterone enanthate as the active ingredient. Testosterone enanthate (EEn) is a Testosterone ester with a longer biological half-life than cypionate. TEn is used commercially in preparations like Delatestryl and Xyosted.

With weekly injection intervals, maximal testosterone serum concentrations up to 78 nmol/l are observed at steady state shortly after injection and minimal testosterone serum concentrations up to 40 nmol/l just before the next injection – meaning you won’t drop below physiological “normal” ranges between doses.

*Due to the extended half-life of Testosterone enanthate, injections at 7-10 days will yield similar results and avoid mood swings and other symptoms associated with the trough. So plan to inject weekly, and give yourself a little grace if you are a day late – it won’t affect the course of your treatment.

Avoiding Contamination: If used correctly, one 10ml vial of Testosterone enanthate can last almost a year (40 weeks of weekly 0.25ml injections). While the Testosterone and carrier oil are sterile and fairly stable in unopened condition, the largest risk for your vial to go bad is from external contamination. External contamination can occur if bacteria or fungi are introduced to the vial from contaminated withdrawal needles, or more likely, contamination building up on the diaphragm, and then pushed into the vial by a sterile needle. This is why it is critical to keep that surface as clean as possible. Additionally, in order to make sure the diaphragm maintains a good seal until the vial is empty, use the smallest needle size possible to withdraw.

Dosage: Effective dosing of Testosterone in transmasculine people is a controversial topic. With the current medical system, blood tests are used as the first measure to adjust dosage in order to reproduce the levels that are common in cis men. This enables a streamlined treatment of patients who experience gender dysphoria, but often fails to address the patient’s individual transition goals.

For people who are unsure about what dosage to start with, we recommend weekly injections of 0.25ml (50mg per week -> ~7mg per day) of TEn. Depending on your metabolism, this will result in steady state levels around 200-500 pg/ml (= 200-500 ng/dL), sufficient to induce medical transition. After 4-6 weeks you will have some feeling for how testosterone affects your mood (before vs after your day of injection). We encourage you to adjust your dosage up or down depending on what feels best for you (up to a reasonable maximum weekly dose of ~0.5ml (100mg). After settling on your personal dose for some weeks, test your blood levels to make sure your dosage is safe and sustainable.

Storage: It is important to keep the vial in a clean environment. If you notice any contamination, please dispose of it.

DOI: 10.1056/NEJMcp1903650

Testosterone: Action, Deficiency, Substitution

4th Edition

ISBN: 9781107012905